Targeted next-generation sequencing (NGS) is often performed using hybridization-based target enrichment, which deploys oligonucleotide probes to capture regions of interest for downstream sequencing.

The ability to label nucleic acids with a variety of tagged molecules has led to astonishing advances in molecular biology. Labeled DNA, RNA, and oligonucleotide probes have made possible techniques ...

PCR has fundamentally changed molecular biology, offering a rapid means to amplify minute quantities of DNA. Real-time quantitative PCR (Q-PCR) techniques are designed to facilitate accurate ...

Use of Adapter Blocking Oligos for In-Solution Hybridization Improves Target Capture Performance In-solution hybridization (target capture) has been used since the early days of next-generation ...

High-resolution melting has proven to be a highly sensitive method for mutation discovery. PCR is performed in the presence of the double-strand DNA binding dye LCGreen® Plus. Samples are then ...